Abstract
The interaction or association of polystyrene microspheres with blood constituents, and the effect on the immune response were studied with blood and tissue samples following i.v. administration of 1-, 3-, and 12-μm spheres into Beagle dogs. One- and 3-μm spheres were found in the lysosomes inside of the cytoplasm of monocytes and granulocytes with a residence time of up to at least 24 hr while the 12-μm spheres were observed outside of the monocytes and granulocytes. There was no evidence of any damage to the lysosomes or other cellular organelles and normal enzymatic function of the monocytes and granulocytes was maintained as evidenced by normal distribution of esterase enzymes in the cells. The effect of the microspheres on serum protein distribution was determined by analysis of protein fractions including gamma globulin between controls and samples and cell-mediated immune response was assessed by measuring the responsiveness of the peripheral blood lymphocytes to mitogenic stimulation. The response of T and B lymphocytes to pokeweed, phytohemagglutinin, and concanavalin A mitogens showed no change 7 days after microsphere administration of the 1- and 12-μrn sizes. The serum protein levels including gamma globulin were unchanged from control values indicating the spheres did not antigenically stimulate the immune response.
- Received April 19, 1983.
- Accepted August 15, 1983.
- Copyright © Parenteral Drug Association. All rights reserved.
PDA members receive access to all articles published in the current year and previous volume year. Institutional subscribers received access to all content. Log in below to receive access to this article if you are either of these.
If you are neither or you are a PDA member trying to access an article outside of your membership license, then you must purchase access to this article (below). If you do not have a username or password for JPST, you will be required to create an account prior to purchasing.
Full issue PDFs are for PDA members only.
Note to pda.org users
The PDA and PDA bookstore websites (www.pda.org and www.pda.org/bookstore) are separate websites from the PDA JPST website. When you first join PDA, your initial UserID and Password are sent to HighWirePress to create your PDA JPST account. Subsequent UserrID and Password changes required at the PDA websites will not pass on to PDA JPST and vice versa. If you forget your PDA JPST UserID and/or Password, you can request help to retrieve UserID and reset Password below.