Abstract
n–alkylpolyoxyethylene surfactants (CnH2n+1O(CH2CH20)mH; CnEm) showed a strong enhancing effect on the inactivation of lipopolysaccharide (LPS) by heat treatment over a wide range of temperatures. The effect of CnE8 (n = 10–16) was observed above the critical micelle concentration (CMC) and above the cloud point, and was influenced by the length of the alkyl chains. The efficacy of the surfactants was in the order C10E8 < C12E8, Cl6 E8 < C!4E8. However, the hydrophilic moiety seemed to have no influence. An 80–95% solution of n–butanol showed a similar effect, indicating that LPS was more effectively inactivated in the oily phase of the surfactants than in water. The effect of surfactant on the hydrodynamic diameter of LPS was the same before and after steam–heat treatment for 20 minutes at 121°C. Each surfactant disaggregated LPS without alteration of the activity of LPS before the heat treatment. We consider that the surfactants interact with LPS in the region of lipid A in a manner that favors loss of the activities of LPS during heating.
- Received August 30, 1996.
- Accepted March 28, 1997.
- Copyright © Parenteral Drug Association. All rights reserved.
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