PT  - JOURNAL ARTICLE
AU  - Angel L. Salaman-Byron
TI  - Probable Scenarios of Process Contamination with <em>Cutibacterium</em> (<em>Propionibacterium</em>) <em>acnes</em> in Mammalian Cell Bioreactor
AID  - 10.5731/pdajpst.2019.010710
DP  - 2020 Sep 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 592--601
VI  - 74
IP  - 5
4099  - http://journal.pda.org/content/74/5/592.short
4100  - http://journal.pda.org/content/74/5/592.full
SO  - PDA J Pharm Sci Technol2020 Sep 01; 74
AB  - Mammalian cell lines constitute an important part in the manufacture of therapeutic proteins. A contamination-free operation of a mammalian cell bioreactor demands constant attention to details. Cutibacterium acnes, a slow-growing bacterium, is a common skin flora and is often associated with mammalian cell bioreactor contaminations. This paper reviews the literature published about such C. acnes contaminations and presents three hypothetical contamination scenarios based on the author’s experience in the hope of fostering early detection of contamination events. Bioreactor process parameters such as unusual fluctuation in dissolved oxygen, high levels of ammonia, and microscopic examination have been identified as useful tools to detect slow-growing bacteria contamination.