RT Journal Article
SR Electronic
T1 Tailored media-fill test protocols inspired by 68Ga kit-based radiopharmaceuticals
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP pdajpst.2021.012657
DO 10.5731/pdajpst.2021.012657
A1 Charlotte Donze
A1 Lea Rubira
A1 Pierre Olivier Kotzki
A1 Emmanuel Deshayes
A1 Cyril Fersing
YR 2022
UL http://journal.pda.org/content/early/2022/02/15/pdajpst.2021.012657.abstract
AB Gallium-68 radiolabeling is an increasingly common activity in radiopharmacy. Single vial cold kits to radiolabel DOTATOC and PSMA-11 with 68Ga were developed, either for manual or automated preparation. Both approaches are very specific and require aseptic compounding skills, raising needs for dedicated training. The aim of this work was to design and implement an integrative media-fill test (MFT) protocol inspired by 68Ga kit-based radiopharmaceuticals for operators qualification, suitable for both manual and automated preparation simulations. Three custom MFT protocols (2 manual and 1 automated simulations) compatible with specific radiopharmacy equipment were designed for operators training. During MFT sessions, the microbiological quality of the working environment was monitored by surface and air sampling. The sensitivity of the tryptic soy broth (TSB) and the influence of the number of punctures performed in each vial on units positivity were also studied. Four operators were evaluated and carried out in triplicate the 3 MFTs. None of the 336 incubated units showed turbidity, although 27.8% of surface samples and 11.1% of air samples were positive (1 to 4 CFU). Over 36 MFT sessions, 15 contaminations of the working area by TSB drops occurred. TSB sensitivity was estimated >3.12 UFC. Units positivity showed a probable relationship with the number of punctures in a vial and, more obviously, with the contamination level of the vial stopper. As a part of a general sterile compounding instruction, these MFT protocols may be useful tools for initial and continuing training of operators carrying out manual and automated 68Ga radiolabeling.