PT  - JOURNAL ARTICLE
AU  - Scott, Allison
AU  - Vanbroekhoven, Ans
AU  - Joossen, Cedric
AU  - Knutsen, Chris
AU  - Govezensky, David
AU  - Anders, Hans-Joachim
AU  - Cannon, James
AU  - Salvas, Joanny
AU  - Dingle, Michael
AU  - Hutchins, Patrick
AU  - Merker, Petra
AU  - Villari, Philip
AU  - Ramsey, Stephanie
AU  - Cundell, Anthony
AU  - Navarro, Victoria
AU  - Franz-Riethdorf, Margit
TI  - Challenges Encountered in the Implementation of Bio-Fluorescent Particle Counting Systems as a Routine Microbial Monitoring Tool
AID  - 10.5731/pdajpst.2021.012726
DP  - 2023 Jan 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 2--9
VI  - 77
IP  - 1
4099  - http://journal.pda.org/content/77/1/2.short
4100  - http://journal.pda.org/content/77/1/2.full
SO  - PDA J Pharm Sci Technol2023 Jan 01; 77
AB  - The transition from traditional growth-based microbial detection methods to continuous bio-fluorescent particle counting methods represents a paradigm shift, because the results will be non-equivalent in terms of microbial counts, and a continuous, rather than periodic, data stream will be available. Bio-fluorescent particle counting technology, a type of rapid microbiological method, uses the detection of the intrinsic fluorescence of microbial cells to enumerate bioburden levels in air or water samples, continuously. The reported unit is commonly referred to as an autofluorescence unit, which is not dependent upon growth, as is the traditional method. The following article discusses challenges encountered when implementing this modern technology, and the perspective from a consortium of four industry working groups on navigating these challenges.