PT - JOURNAL ARTICLE AU - Marasa, B. AU - Gaoh, S. Daddy AU - Alusta, P. AU - Lee, Y-.J. AU - LiPuma, J.J. AU - Hussong, D. AU - Ahn, Y. TI - A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms AID - 10.5731/pdajpst.2024.99902 DP - 2024 Nov 01 TA - PDA Journal of Pharmaceutical Science and Technology PG - 753--754 VI - 78 IP - 6 4099 - http://journal.pda.org/content/78/6/753.short 4100 - http://journal.pda.org/content/78/6/753.full SO - PDA J Pharm Sci Technol2024 Nov 01; 78 AB - In pharmaceutical manufacturing, benefit is conferred in detection of specified microorganism (i.e., Burkholderia cepacia complex (BCC), E. coli, Pseudomonas aeruginosa, Salmonella enterica) not readily identified by culture-dependent methods. It's logical to test for the presence of “specified microorganism” using metagenomic analysis before culturing a “specified organism”, especially when the organism isn't easy to culture. We developed a metagenomic analysis during enrichment to identify specified organisms. The enriched bacterial community consisted predominantly of Bacillus spp. and Stenotrophomonas spp., each contributing about 97-99% to total taxon abundance in TSB and 1/10× TSB. The specified microorganisms that were observed were Clostridium spp., Burkholderia spp., and Staphylococcus spp. (0.04 - 0.07%) in TSB, otherwise Burkholderia spp., Pseudomonas spp., Salmonella spp., Staphylococcus spp. and Escherichia spp. (0.01 - 1.73%) in 1/10× TSB. PreQ0 biosynthesis (PWY-6703) and guanosine ribonucleotides de novo biosynthesis (PWY-7221) were the most abundant pathways in 1/10× TSB-24 h. BCC chiefly contributed to the toluene degradation (PWY-5180 and PWY-5182) pathways. Initial results demonstrate the potential of the metagenomic approach during enrichment in water-based environments. These results indicate that a metagenomic enrichment approach to evaluating water samples can be useful to monitor specified organisms over time, including oligotrophs such as BCC in 1/10× TSB.