PT  - JOURNAL ARTICLE
AU  - Reich, R. R.
TI  - <em>Bacillus stearothermophilus</em> Spore Suspensions: Effect of Storage Conditions and Time on Viability and Moist Heat Resistance
DP  - 1981 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 74--78
VI  - 35
IP  - 2
4099  - http://journal.pda.org/content/35/2/74.short
4100  - http://journal.pda.org/content/35/2/74.full
SO  - PDA J Pharm Sci Technol1981 Mar 01; 35
AB  - Storage conditions were shown to affect the viability and moist heat resistance of Bacillus stearothermophilus spore suspensions. Refrigerated storage (4 °C) in distilled water did not significantly affect spore viability, but had an adverse effect on moist heat resistance. Freezer storage (–10 °C) and the subsequent thawings and refreezing of the suspensions was shown to have a significant effect on spore viability without a concomitant loss of moist heat resistance in the surviving population. Refrigerated storage (4 °C) in 0.02 M calcium acetate, pH 9.7, stabilized spore resistance without a significant loss of viability for a minimum of two years.