RT Journal Article SR Electronic T1 Bacillus stearothermophilus Spore Suspensions: Effect of Storage Conditions and Time on Viability and Moist Heat Resistance JF PDA Journal of Pharmaceutical Science and Technology JO PDA J Pharm Sci Technol FD Parenteral Drug Association (PDA) SP 74 OP 78 VO 35 IS 2 A1 Reich, R. R. YR 1981 UL http://journal.pda.org/content/35/2/74.abstract AB Storage conditions were shown to affect the viability and moist heat resistance of Bacillus stearothermophilus spore suspensions. Refrigerated storage (4 °C) in distilled water did not significantly affect spore viability, but had an adverse effect on moist heat resistance. Freezer storage (–10 °C) and the subsequent thawings and refreezing of the suspensions was shown to have a significant effect on spore viability without a concomitant loss of moist heat resistance in the surviving population. Refrigerated storage (4 °C) in 0.02 M calcium acetate, pH 9.7, stabilized spore resistance without a significant loss of viability for a minimum of two years.