PT  - JOURNAL ARTICLE
AU  - Jones, A. T.
AU  - Pflug, I. J.
TI  - <em>Bacillus coagulans</em>, FRR B666, as a Potential Biological Indicator Organism
DP  - 1981 May 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 82--87
VI  - 35
IP  - 3
4099  - http://journal.pda.org/content/35/3/82.short
4100  - http://journal.pda.org/content/35/3/82.full
SO  - PDA J Pharm Sci Technol1981 May 01; 35
AB  - Bacillus coagulans, FRR B666, was evaluated as a potential biological indicator organism. The organism was grown on two types of sporulation media and the spores were tested for wet heat resistance. Further tests were carried out to determine if replicate spore crops exhibited similar heat resistance characteristics. Survivor curve tests were carried out to determine the behavior of B. coagulans spores when heated in Water for Injection (USP), 0.0153 M Butterfield’s buffer, and three representative parenteral solutions. Large numbers of spores were produced using relatively simple sporulation media. Aliquots of replicate spore crops grown on the same lot of medium had almost identical heat destruction characteristics. The semilogarithmic survivor curves in most solutions were straight lines. The results indicate that B. coagulans, FRR B666, is a stable and reliable biological indicator organism. Reproducibility of survivor curves and the low variability among replicate samples in all solutions tested are characteristics that suggest that B. coagulans has attributes of a potentially excellent biological indicator organism.