%0 Journal Article %A Stephen Paul Denyer %A Kathryn Helen Ward %T A Rapid Method for the Detection of Bacterial Contaminants in Intravenous Fluids Using Membrane Filtration and Epifluorescence Microscopy %D 1983 %J PDA Journal of Pharmaceutical Science and Technology %P 156-159 %V 37 %N 5 %X Membrane filtration and epifluorescence (incident light fluorescence) microscopy was used for the rapid detection and enumeration of bacteria in deliberately contaminated intravenous infusion fluids. The technique described is simple to perform, takes less than 30 min from filtration to the completion of analysis, and is sufficiently sensitive to detect contamination levels of as low as 25 organisms/ml. A linear relationship exists between fluorescence counts and pour plate colony counts over the range 25–130,000 organisms/ml (correlation coefficient = 0.991). %U https://journal.pda.org/content/pdajpst/37/5/156.full.pdf