RT Journal Article
SR Electronic
T1 A Rapid Method for the Detection of Bacterial Contaminants in Intravenous Fluids Using Membrane Filtration and Epifluorescence Microscopy
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 156
OP 159
VO 37
IS 5
A1 Denyer, Stephen Paul
A1 Ward, Kathryn Helen
YR 1983
UL http://journal.pda.org/content/37/5/156.abstract
AB Membrane filtration and epifluorescence (incident light fluorescence) microscopy was used for the rapid detection and enumeration of bacteria in deliberately contaminated intravenous infusion fluids. The technique described is simple to perform, takes less than 30 min from filtration to the completion of analysis, and is sufficiently sensitive to detect contamination levels of as low as 25 organisms/ml. A linear relationship exists between fluorescence counts and pour plate colony counts over the range 25–130,000 organisms/ml (correlation coefficient = 0.991).