PT  - JOURNAL ARTICLE
AU  - Prior, C. P.
AU  - Doyle, K. R.
AU  - Duffy, S. A.
AU  - Hope, J. A.
AU  - Moellering, B. J.
AU  - Prior, G. M.
AU  - Scott, R. W.
AU  - Tolbert, W. R.
TI  - The Recovery of Highly Purified Biopharmaceuticals from Perfusion Cell Culture Bioreactors
DP  - 1989 Jan 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 15--23
VI  - 43
IP  - 1
4099  - http://journal.pda.org/content/43/1/15.short
4100  - http://journal.pda.org/content/43/1/15.full
SO  - PDA J Pharm Sci Technol1989 Jan 01; 43
AB  - The impact of continuous perfusion cell culture technology on production of one and two chain human rtPA, as well as monoclonal IgG and IgM, will be reviewed. Perfusion as compared to batch systems improve the quality of the product in the conditioned media in terms of biological activity and structural integrity. This significantly increases downstream recovery and daily production output of final purified material. These findings are a consequence of the ability of perfusion technology to 1) maintain cells intact at high cell density; 2) effectively reduce serum content to approximate serum free conditions; and 3) minimize residency time of labile product within the 37°C environment of the bioreactor.