PT  - JOURNAL ARTICLE
AU  - Tsuji, Kiyoshi
TI  - Expert System Computer Program for Troubleshooting the Gel-Clot Limulus Amebocyte Lysate (LAL) Assay
DP  - 1989 Sep 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 240--245
VI  - 43
IP  - 5
4099  - http://journal.pda.org/content/43/5/240.short
4100  - http://journal.pda.org/content/43/5/240.full
SO  - PDA J Pharm Sci Technol1989 Sep 01; 43
AB  - This article describes a knowledge-based or expert system computer program that can assist in troubleshooting the gel-clot Limulus Amebocyte Lysate (LAL) method for the assay of endotoxin in parenteral products and in-process materials. The program may also be used to train new personnel and/or to assist in the development of an LAL method for a new product or material. The program includes LAL assay information for 31 parenteral products and materials. The program uses rules-of-thumb and intuition to diagnose 155 possible LAL assay problems and logically reduce them to 37 recommended problem solving processes. The program also provides references to support its recommendation.