PT  - JOURNAL ARTICLE
AU  - P. A. Martin
AU  - G. L. Gaunnac
AU  - K. Tsuji
TI  - Automation of Microtiter Plate-Chromogenic Substrate LAL Endotoxin Assay Method by Use of a Modified Cetus Pro/Pette Express System
DP  - 1986 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 61--65
VI  - 40
IP  - 2
4099  - http://journal.pda.org/content/40/2/61.short
4100  - http://journal.pda.org/content/40/2/61.full
SO  - PDA J Pharm Sci Technol1986 Mar 01; 40
AB  - An automated microtiter plate-chromogenic substrate LAL system for the assay of bacterial endotoxin has been developed using a Cetus Pro/Pette EXPRESS® system, modified with the addition of a solid-state incubator block. The automated system assays 96 samples in approximately 22 min. Sensitivity of the automated system is approximately 0.01 EU/ml with a linear assay range from 0.03 to 0.14 EU/ml and the relative standard deviation (RSD) of approximately 6%. The automated microtiter plate-chromogenic substrate LAL system has been validated for testing a variety of pharmaceutical injectable products and materials. The automated system has been shown to represent significant improvements in speed, accuracy, and precision of the LAL assay method for endotoxin.