RT Journal Article
SR Electronic
T1 Airborne Bacteria Sampling: The Effect of Dehydration and Sampling Time
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 182
OP 188
VO 40
IS 5
A1 Whyte, W.
A1 Niven, L.
YR 1986
UL http://journal.pda.org/content/40/5/182.abstract
AB Lengthy airborne sampling is thought to cause desiccation of bacterial media and loss of viability of collected bacteria. Experiments reported in this paper show that micro-organisms commonly found in room air, are little affected (approximately 8% reduction in viability) by a 13% reduction in the water content of the agar inpetri dishes. Acceptance of this reduction in viability would allow bacterial plates to be exposed for about 24 hr in still room air, over 6 hr in a laminar-flow work bench, and over 1 hr in a 30 liters/min (7 ft3/min) bacterial slit sampler. It is demonstrated that to obtain accurate air sampling results in clean areas, air sampling times must be extended considerably from what is now, common practice.