PT  - JOURNAL ARTICLE
AU  - Avis, Kenneth E.
AU  - Jewell, Roxanne C.
AU  - Ludwig, John D.
TI  - Studies on the Thermal Destruction of Escherichia Coli Endotoxin
DP  - 1987 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 49--56
VI  - 41
IP  - 2
4099  - http://journal.pda.org/content/41/2/49.short
4100  - http://journal.pda.org/content/41/2/49.full
SO  - PDA J Pharm Sci Technol1987 Mar 01; 41
AB  - The dry heat destruction of Escherichia coli endotoxin in Type I glass vials was studied at three oven operating temperatures, namely, 175 °C, 210 °C, and 250 °C, utilizing typical processing conditions in both a connective and a radiant heating oven. Also, five different lots of E. coli endotoxin were compared, three of which were a standard commercial endotoxin and two were a purified lipopolysaccharide. A total of 5265 samples of endotoxin were tested using the Limulus Amebocyte Lysate gel endpoint test, having a sensitivity of 0.005 ng/0.1 ml. It was found that destruction of endotoxin occurred in a time equivalent to F54170 values at 130,150, and 1800 min, respectively, for the three temperatures utilized.