RT Journal Article
SR Electronic
T1 Studies on the Thermal Destruction of Escherichia Coli Endotoxin
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 49
OP 56
VO 41
IS 2
A1 Avis, Kenneth E.
A1 Jewell, Roxanne C.
A1 Ludwig, John D.
YR 1987
UL http://journal.pda.org/content/41/2/49.abstract
AB The dry heat destruction of Escherichia coli endotoxin in Type I glass vials was studied at three oven operating temperatures, namely, 175 °C, 210 °C, and 250 °C, utilizing typical processing conditions in both a connective and a radiant heating oven. Also, five different lots of E. coli endotoxin were compared, three of which were a standard commercial endotoxin and two were a purified lipopolysaccharide. A total of 5265 samples of endotoxin were tested using the Limulus Amebocyte Lysate gel endpoint test, having a sensitivity of 0.005 ng/0.1 ml. It was found that destruction of endotoxin occurred in a time equivalent to F54170 values at 130,150, and 1800 min, respectively, for the three temperatures utilized.