PT  - JOURNAL ARTICLE
AU  - Paul Denyer, Stephen
AU  - Lynn, Richard
TI  - A Sensitive Method for the Rapid Detection of Bacterial Contaminants in Intravenors Fluids
DP  - 1987 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 60--66
VI  - 41
IP  - 2
4099  - http://journal.pda.org/content/41/2/60.short
4100  - http://journal.pda.org/content/41/2/60.full
SO  - PDA J Pharm Sci Technol1987 Mar 01; 41
AB  - Memberane Filtration and epiflurescence microscopy were combined with a sample incubation stage to enable the detection of low levels of bacterial contaminantes.The device and technique described allows the detection of as few as six viable organisms within a total incubation and analysis time of 6 hr,irrespective of sample volume.Linear relationships (corrlation coeficients ≥ 0.940)were shown to exist between post-incubation membrane fluorescence counts and initial pour plate colony counts for both vegetation and spore-forming bacteria and for all incubation times tested. The technique was successfully applied to the detection and enumeration of a mixed flora in normal saline and of survivours in a phenol-preserved.solution