RT Journal Article
SR Electronic
T1 A Sensitive Method for the Rapid Detection of Bacterial Contaminants in Intravenors Fluids
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 60
OP 66
VO 41
IS 2
A1 Paul Denyer, Stephen
A1 Lynn, Richard
YR 1987
UL http://journal.pda.org/content/41/2/60.abstract
AB Memberane Filtration and epiflurescence microscopy were combined with a sample incubation stage to enable the detection of low levels of bacterial contaminantes.The device and technique described allows the detection of as few as six viable organisms within a total incubation and analysis time of 6 hr,irrespective of sample volume.Linear relationships (corrlation coeficients ≥ 0.940)were shown to exist between post-incubation membrane fluorescence counts and initial pour plate colony counts for both vegetation and spore-forming bacteria and for all incubation times tested. The technique was successfully applied to the detection and enumeration of a mixed flora in normal saline and of survivours in a phenol-preserved.solution