RT Journal Article
SR Electronic
T1 A Novel Method of Assessment of Interfacial Adsorption of Blood Proteins
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 239
OP 245
VO 45
IS 5
A1 Burgess, Diane J.
A1 Longo, Luigi
A1 Yoon, Joseph K.
YR 1991
UL http://journal.pda.org/content/45/5/239.abstract
AB Interfacial adsorption of proteins can be studied using microscopic surface analysis techniques, and by physical techniques such as interfacial tension measurement. A novel method of surface analysis of adsorbed protein layers is described, which involves interfacial shear rheology of aqueous solutions of proteins. This technique provides information on the structural-mechanical properties of the adsorbed layers which may be related to: the rate of interfacial adsorption, interfacial interactions, and conformational changes in the adsorbed layers. The interfacial shear rheology of aqueous solutions of the blood proteins, bovine serum albumin and human immunoglobulin G was investigated at the air/aqueous interface. The effects of bulk concentration (0.1 to 4.0% w/v) and pH (3 to 8) were investigated, both interfacial viscosity and elasticity values are reported.