PT  - JOURNAL ARTICLE
AU  - Lois K. Schadewald
AU  - D. G. Martin
AU  - Kiyoshi Tsuji
TI  - Automation and Computerization of Chromogenic LAL Assay Method for Bacterial Endotoxin Using 96-Well Microtiter Plate
DP  - 1990 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 50--53
VI  - 44
IP  - 2
4099  - http://journal.pda.org/content/44/2/50.short
4100  - http://journal.pda.org/content/44/2/50.full
SO  - PDA J Pharm Sci Technol1990 Mar 01; 44
AB  - A high degree of automation was achieved for a chromogenic substrate Limulus amebocyte lysate (LAL) assay method for bacteria! endotoxin using a Cetus Pro/Group Liquid Handler and 96-well microliter plates. A Titertek Multiskan Reader was interfaced with an IBM PC using a LOTUS MEASURE software to capture optical density values of samples in a LOTUS 1-2-3 spreadsheet. A password protected, menu-driven macro programmed in LOTUS 1-2-3 automates the calculation, evaluation of assay parameters, documentation, and generation of a formatted three-page report suitable as a primary record. All assay operations, including testing 19 samples against a four-point standard curve in replicates of four each, the calculation of results, and generation of a report, are completed in less than 40 minutes. The relative standard deviation (RSD) of the assay is approximately 7%, which compares favorably with the robot automated system.