RT Journal Article
SR Electronic
T1 Automation and Computerization of Chromogenic LAL Assay Method for Bacterial Endotoxin Using 96-Well Microtiter Plate
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 50
OP 53
VO 44
IS 2
A1 Lois K. Schadewald
A1 D. G. Martin
A1 Kiyoshi Tsuji
YR 1990
UL http://journal.pda.org/content/44/2/50.abstract
AB A high degree of automation was achieved for a chromogenic substrate Limulus amebocyte lysate (LAL) assay method for bacteria! endotoxin using a Cetus Pro/Group Liquid Handler and 96-well microliter plates. A Titertek Multiskan Reader was interfaced with an IBM PC using a LOTUS MEASURE software to capture optical density values of samples in a LOTUS 1-2-3 spreadsheet. A password protected, menu-driven macro programmed in LOTUS 1-2-3 automates the calculation, evaluation of assay parameters, documentation, and generation of a formatted three-page report suitable as a primary record. All assay operations, including testing 19 samples against a four-point standard curve in replicates of four each, the calculation of results, and generation of a report, are completed in less than 40 minutes. The relative standard deviation (RSD) of the assay is approximately 7%, which compares favorably with the robot automated system.