PT  - JOURNAL ARTICLE
AU  - Laska, Dennis A.
AU  - Williams, Patricia D.
AU  - Reboulet, John T.
AU  - Morris, Robert M.
TI  - The L6 Muscle Cell Line as a Tool to Evaluate Parenteral Products for Irritation
DP  - 1991 Mar 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 77--82
VI  - 45
IP  - 2
4099  - http://journal.pda.org/content/45/2/77.short
4100  - http://journal.pda.org/content/45/2/77.full
SO  - PDA J Pharm Sci Technol1991 Mar 01; 45
AB  - A rat skeletal muscle cell line (L6) was evaluated for its utility in assessing cellular damage caused by parenteral administration of eight commercially available pharmaceuticals. The physical forms of the eight pharmaceuticals were diverse, including aqueous and non-aqueous suspensions and solutions. The L6 cells were exposed to therapeutic as well as diluted concentrations of methocarbamol, lorazepam, dimercaprol, phytonadione, menadiol sodium phosphate, penicillin G procaine, penicillin G benzathine, and iron dextran complex. Irritation assessment was based on the depletion of creatine phosphokinase (CK)from treated cultures versus untreated controls. The results obtained correlate well with reports of irritation and side effects noted in clinical use, and demonstrate the versatility of the model for testing suspensions and nonaqueous parenteral solutions. The L6 model is a useful tool to assist in determining the relative local irritancy of parenteral products.