PT  - JOURNAL ARTICLE
AU  - Esteban, Jose Maria
AU  - Diaz, Mariano Leiva
AU  - Soriano, Fabian
AU  - Marin, Enrique
TI  - Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions
DP  - 1992 Sep 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 146--149
VI  - 46
IP  - 5
4099  - http://journal.pda.org/content/46/5/146.short
4100  - http://journal.pda.org/content/46/5/146.full
SO  - PDA J Pharm Sci Technol1992 Sep 01; 46
AB  - Epifluorescence microscopy of the microcolonies obtained after filtration of a deliberately contaminated intravenous solution, was used for the rapid detection and enumeration of the bacteria. The technique is adequate for the test of low volume solution (with low contamination). Also it allows one to detect a single colony forming unit (c.f.u.)/mL, with great correlation between fluorescence counts and pour plate colony counts; the morphology of microcolonies is typical for most of the microorganisms and it allows a primoidentification (for a trained analist).