TY - JOUR T1 - Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions JF - PDA Journal of Pharmaceutical Science and Technology JO - PDA J Pharm Sci Technol SP - 146 LP - 149 VL - 46 IS - 5 AU - Jose Maria Esteban AU - Mariano Leiva Diaz AU - Fabian Soriano AU - Enrique Marin Y1 - 1992/09/01 UR - http://journal.pda.org/content/46/5/146.abstract N2 - Epifluorescence microscopy of the microcolonies obtained after filtration of a deliberately contaminated intravenous solution, was used for the rapid detection and enumeration of the bacteria. The technique is adequate for the test of low volume solution (with low contamination). Also it allows one to detect a single colony forming unit (c.f.u.)/mL, with great correlation between fluorescence counts and pour plate colony counts; the morphology of microcolonies is typical for most of the microorganisms and it allows a primoidentification (for a trained analist). ER -