RT Journal Article
SR Electronic
T1 Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 146
OP 149
VO 46
IS 5
A1 Esteban, Jose Maria
A1 Diaz, Mariano Leiva
A1 Soriano, Fabian
A1 Marin, Enrique
YR 1992
UL http://journal.pda.org/content/46/5/146.abstract
AB Epifluorescence microscopy of the microcolonies obtained after filtration of a deliberately contaminated intravenous solution, was used for the rapid detection and enumeration of the bacteria. The technique is adequate for the test of low volume solution (with low contamination). Also it allows one to detect a single colony forming unit (c.f.u.)/mL, with great correlation between fluorescence counts and pour plate colony counts; the morphology of microcolonies is typical for most of the microorganisms and it allows a primoidentification (for a trained analist).