TY - JOUR T1 - Mapping Size Distributions of Parenteral Fluorocarbon Emulsions by a Novel Method of Centrifugal Sedimentation Followed by Densitometry JF - PDA Journal of Pharmaceutical Science and Technology JO - PDA J Pharm Sci Technol SP - 233 LP - 238 VL - 46 IS - 6 AU - shireesh P. Apte AU - Salvatore J. Turco Y1 - 1992/11/01 UR - http://journal.pda.org/content/46/6/233.abstract N2 - A novel method for characterization of size distributions of parenteral fluorocarbon emulsions is described. Prepared emulsions were centrifuged to sediment droplets above predetermined diameters out of a known supernatant sample volume using the Bostok-Stoke’s equation. Centrifugation times may be calculated using centrifuge parameters and physical properties of the fluorocarbon oil phase and the dispersion medium. The fluorocarbon content of the supernatant sample volume at successive centrifugation times was determined both by densitometry and by Gas Chromatography. A close correlation was found between the two methods. The density data was processed and converted into a volume distribution histogram by means of a program written in BASIC. The speed, simplicity of use, non reliance on costly equipment and good correlation to absolute particle counting methods makes the density method suitable for submicron size characterization. ER -