RT Journal Article
SR Electronic
T1 Mapping Size Distributions of Parenteral Fluorocarbon Emulsions by a Novel Method of Centrifugal Sedimentation Followed by Densitometry
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 233
OP 238
VO 46
IS 6
A1 shireesh P. Apte
A1 Salvatore J. Turco
YR 1992
UL http://journal.pda.org/content/46/6/233.abstract
AB A novel method for characterization of size distributions of parenteral fluorocarbon emulsions is described. Prepared emulsions were centrifuged to sediment droplets above predetermined diameters out of a known supernatant sample volume using the Bostok-Stoke’s equation. Centrifugation times may be calculated using centrifuge parameters and physical properties of the fluorocarbon oil phase and the dispersion medium. The fluorocarbon content of the supernatant sample volume at successive centrifugation times was determined both by densitometry and by Gas Chromatography. A close correlation was found between the two methods. The density data was processed and converted into a volume distribution histogram by means of a program written in BASIC. The speed, simplicity of use, non reliance on costly equipment and good correlation to absolute particle counting methods makes the density method suitable for submicron size characterization.