PT  - JOURNAL ARTICLE
AU  - Nema, Sandeep
AU  - Kiick, Dennis M.
AU  - Avis, Kenneth E.
TI  - Protection of Lactate Dehydrogenase Against Protease Digestion by Encapsulation in Liposomes
DP  - 1994 Sep 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 231--235
VI  - 48
IP  - 5
4099  - http://journal.pda.org/content/48/5/231.short
4100  - http://journal.pda.org/content/48/5/231.full
SO  - PDA J Pharm Sci Technol1994 Sep 01; 48
AB  - Lactate dehydrogenase (LDH) was entrapped in phosphatidylcholine liposomes to evaluate the protective effect of liposomes against protease digestion. Three different preparations of LDH either encapsulated in liposomes, unencapsulated in liposomes or in the absence of liposomes were incubated with the protease trypsin. The loss of LDH activity was measured at intervals over a 12-hour period. The degradation rate of LDH was found to be the same when LDH was unencapsulated in the presence or absence of liposomes. However, when LDH was entrapped in liposomes the degradation rate was 4 to 24 folds slower. This finding indicates that encapsulation of a protein in a liposome protects the protein from the degrading effects of a protease enzyme.