RT Journal Article
SR Electronic
T1 Protection of a Model Protein, Lactate Dehydrogenase, by Encapsulation in Liposome
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 213
OP 218
VO 50
IS 4
A1 Sandeep Nema
A1 Kenneth E. Avis
YR 1996
UL http://journal.pda.org/content/50/4/213.abstract
AB The objective of this study was to determine if encapsulating a model protein, lactate dehydrogenase (LDH), inside liposomes can protect it during storage, upon freeze-thaw cycles and during freeze-drying. LDH, which undergoes cold denaturation, was entrapped insides liposomes prepared using modification of Bangham's method. It was found that when LDH was entrapped in liposomes and subjected to a single freeze-thaw treatment, there was about 7 times more recovery of activity than when LDH was free in solution. Similarly, LDH encapsulated in liposomes showed 60% recovery after freeze-drying compared to 1–2% recovery for the unentrapped LDH. The role of phospholipid content, residual moisture content and protein content were evaluated. Possible mechanism(s) of cold temperature inactivation of proteins and role of liposomes in preventing such cryodenaturation will be discussed in light of these results.