TY - JOUR T1 - Enhancing Effect of Non-ionic Surfactant on the Inactivation of Lipopolysaccharide by Steam-Heat Treatment I JF - PDA Journal of Pharmaceutical Science and Technology JO - PDA J Pharm Sci Technol SP - 360 LP - 365 VL - 50 IS - 6 AU - Takaaki Bamba AU - Rakan Matsui AU - Kazuo Watabe Y1 - 1996/11/01 UR - http://journal.pda.org/content/50/6/360.abstract N2 - Polyoxyethylene (20) sorbitan mono-fatty acid esters strongly enhanced the inactivation of lipopolysaccharide (LPS) by steam-heat treatment at 121°C, as assayed by using the Limulus amebocyte lysate (LAL) and the pyrogen test. In an aqueous solution containing 0.1% surfactant, the decrease of LPS (1 µg/ml)from E. coli 055:B5 at 121°C followed first-order kinetics. Based on the LAL assay, 0.1% surfactant was essential to achieve 3-log cycle reduction of LPS with concomitant loss of pyrogenicity by steam-heat treatment for 20 min at 121°C. Steam-heat treatment for 20 min at 121°C in the absence of surfactant was insufficient to achieve depyrogenation. Polyoxyethylene (9) lauryl ether and decaglycerin mono-laurate similarly enhanced depyrogenation by steam-heat treatment. The effects of all the surfactants were concentration-dependent for all of the six kinds of LPS examined. ER -