PT  - JOURNAL ARTICLE
AU  - Sasaki, Tsuguo
AU  - Nishiyama, Takeo
AU  - Shintani, Miharu
AU  - Kenri, Tsuyoshi
TI  - Evaluation of A New Method for Identification of Bacteria Based on Sequence Homology of 16S rRNA Gene
DP  - 1997 Nov 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 242--247
VI  - 51
IP  - 6
4099  - http://journal.pda.org/content/51/6/242.short
4100  - http://journal.pda.org/content/51/6/242.full
SO  - PDA J Pharm Sci Technol1997 Nov 01; 51
AB  - A new identification method for bacteria based on partial sequences of divergent regions of the 16S rRNA gene was evaluated. The method involves PCR-based amplification of 16S rRNA gene fragments, followed by sequencing and comparison of sequences of about 300 nucleotides with those in the database of NCBI (National Center for Biotechnology Information) via the Internet. Most of the bacteria tested could be identified at the species level even if some unread nucleotides were present in the sequence. Although this method still requires improvement, it has the potential to be a highly reliable and practical identification method for bacteria.