PT  - JOURNAL ARTICLE
AU  - Sarbolouki, M. N.
AU  - Toliat, T.
TI  - Storage Stability of Stabilized MLV and REV Liposomes Containing Sodium Methotrexate (Aqueous & Lyophilized)
DP  - 1998 Jan 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 23--27
VI  - 52
IP  - 1
4099  - http://journal.pda.org/content/52/1/23.short
4100  - http://journal.pda.org/content/52/1/23.full
SO  - PDA J Pharm Sci Technol1998 Jan 01; 52
AB  - Multilamellar and unilamellar liposomes containing sodium methotrexate (MTX) were prepared by thin film hydration and reverse-phase evaporation methods. Encapsulation efficiency and release of MTX from liposomes at 4°C, 25°C and 37°C were determined. When stored at 25°C the liposomes lost 33% MTX after one month. To stabilize liposomes, they were freeze-dried in presence of different amounts of cryoprotectants (trehalose, PEG 10,000, PEG 20,000, and a mixture of trehalose-PEG 20,000). Measurements showed that the mixture of trehalose-PEG 20,000 imparted an MTX retention of about 70% after lyophilization. Stability tests of the lyophilized product at 4°C, 25°C and 37°C demonstrated they were fairly stable even at 37°C, indicating their potential use in commercial applications.