RT Journal Article
SR Electronic
T1 Storage Stability of Stabilized MLV and REV Liposomes Containing Sodium Methotrexate (Aqueous & Lyophilized)
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 23
OP 27
VO 52
IS 1
A1 Sarbolouki, M. N.
A1 Toliat, T.
YR 1998
UL http://journal.pda.org/content/52/1/23.abstract
AB Multilamellar and unilamellar liposomes containing sodium methotrexate (MTX) were prepared by thin film hydration and reverse-phase evaporation methods. Encapsulation efficiency and release of MTX from liposomes at 4°C, 25°C and 37°C were determined. When stored at 25°C the liposomes lost 33% MTX after one month. To stabilize liposomes, they were freeze-dried in presence of different amounts of cryoprotectants (trehalose, PEG 10,000, PEG 20,000, and a mixture of trehalose-PEG 20,000). Measurements showed that the mixture of trehalose-PEG 20,000 imparted an MTX retention of about 70% after lyophilization. Stability tests of the lyophilized product at 4°C, 25°C and 37°C demonstrated they were fairly stable even at 37°C, indicating their potential use in commercial applications.