PT  - JOURNAL ARTICLE
AU  - L.G. Piluso
AU  - M.Y. Martinez
TI  - Resolving Liposomal Inhibition of Quantitative LAL Methods
DP  - 1999 Sep 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 260--263
VI  - 53
IP  - 5
4099  - http://journal.pda.org/content/53/5/260.short
4100  - http://journal.pda.org/content/53/5/260.full
SO  - PDA J Pharm Sci Technol1999 Sep 01; 53
AB  - Bacterial endotoxin is composed of Lipid A as well as variable polysaccharide regions, and is capable of eliciting a pyrogenic response in vivo (1). NeXstar has developed a quantitative LAL assay at maximum sensitivity to provide assurance that its products are well below the endotoxin pyrogenic threshold. This test uses rigorous treatment of 1.0mL liposome samples with sodium dodecyl sulfate, an anionic surfactant, or CHAPS, a zwitterionic surfactant to disrupt the lipids present in the liposome sample including endotoxin. This liposome disruption allows potentially undetectable endotoxin to become available to the LAL cascade for quantification and overcomes inhibition of the LAL test by liposomes.