PT  - JOURNAL ARTICLE
AU  - Viegas, Tacey X.
AU  - Van Winkle, Lise L.
TI  - Preformulation Studies for the Development of a Parenteral Liquid Formulation of the Immunomodulator, Peldesine
DP  - 1999 Nov 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 303--308
VI  - 53
IP  - 6
4099  - http://journal.pda.org/content/53/6/303.short
4100  - http://journal.pda.org/content/53/6/303.full
SO  - PDA J Pharm Sci Technol1999 Nov 01; 53
AB  - Peldesine (BCX-34) is a potent inhibitor of the enzyme purine nucleoside phosphorylase (PNP). The object of this study was to determine the preformulation parameters of BCX-34 and subsequently prepare a sterile liquid formulation for intravenous infusion. Ionization and solubility parameters were assessed prior to formulation development. Three pKa values of approximately 4.0, 5.2 and 10.3 were assigned for BCX-34. The pH-solubility profile showed an increase in solubility when the pH of the solution was less than the pKa1 value of 4.0 and when the pH was more than the pKa3 value of 10.3. BCX-34 has an octanol-water partition coefficient of approximately 7:1 (Log P = 0.8). The Van't Hoff temperature dependent solubility when measured over a range of 4 - 40°C produced a heat of solution (ΔHc) of 7.68 kcal/mole. Based on these preformulation observations, two parenteral formulations containing 1 and 5 mg/mL of BCX-34 were prepared in an acidified saline solution and filled into 30cc and 100cc glass vials. Both formulation batches were sterile filtered, while only one 1 mg/mL batch was terminally autoclaved. The long-term stability of both solutions was performed and compared. Results of this study show that the drug product was stable. Following a screening study, Lactated Ringer's injection USP was selected as a suitable infusion medium for the dilution of the drug concentrate solutions.