PT  - JOURNAL ARTICLE
AU  - Benoît Ramond
AU  - Xavier Rolland
AU  - Cécile Planchez
AU  - Philippe Cornet
AU  - Clarisse Antoni
AU  - Jean-Louis Drocourt
TI  - Enumeration of Total Viable Microorganisms in an Antibiotic Raw Material Using Chem<em>Scan</em>® Solid Phase Cytometer
DP  - 2000 Jul 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 320--331
VI  - 54
IP  - 4
4099  - http://journal.pda.org/content/54/4/320.short
4100  - http://journal.pda.org/content/54/4/320.full
SO  - PDA J Pharm Sci Technol2000 Jul 01; 54
AB  - A preliminary study was performed for the enumeration of microorganism contamination of the macrolide antibiotic, Spiramycin®, using epifluorescence with the ChemScan® solid phase cytometer. The artificial spiking of Spiramycin powder antibiotic with pure culture of four microorganisms led to complete recovery of the tested organisms, whatever their sensitivity to the bacteriostatic activity of the drug. With the conventional plate method run in parallel, complete recovery was only obtained for Spiramycin resistant organisms. The spiked microorganisms that were sensitive to the antibiotic remained inhibited or stressed by the action of the Spiramycin and did not grow on the plate.