RT Journal Article
SR Electronic
T1 Enumeration of Total Viable Microorganisms in an Antibiotic Raw Material Using Chem<em>Scan</em>® Solid Phase Cytometer
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 320
OP 331
VO 54
IS 4
A1 Ramond, Benoît
A1 Rolland, Xavier
A1 Planchez, Cécile
A1 Cornet, Philippe
A1 Antoni, Clarisse
A1 Drocourt, Jean-Louis
YR 2000
UL http://journal.pda.org/content/54/4/320.abstract
AB A preliminary study was performed for the enumeration of microorganism contamination of the macrolide antibiotic, Spiramycin®, using epifluorescence with the ChemScan® solid phase cytometer. The artificial spiking of Spiramycin powder antibiotic with pure culture of four microorganisms led to complete recovery of the tested organisms, whatever their sensitivity to the bacteriostatic activity of the drug. With the conventional plate method run in parallel, complete recovery was only obtained for Spiramycin resistant organisms. The spiked microorganisms that were sensitive to the antibiotic remained inhibited or stressed by the action of the Spiramycin and did not grow on the plate.