TY - JOUR T1 - Enumeration of Total Viable Microorganisms in an Antibiotic Raw Material Using Chem<em>Scan</em>® Solid Phase Cytometer JF - PDA Journal of Pharmaceutical Science and Technology JO - PDA J Pharm Sci Technol SP - 320 LP - 331 VL - 54 IS - 4 AU - Benoît Ramond AU - Xavier Rolland AU - Cécile Planchez AU - Philippe Cornet AU - Clarisse Antoni AU - Jean-Louis Drocourt Y1 - 2000/07/01 UR - http://journal.pda.org/content/54/4/320.abstract N2 - A preliminary study was performed for the enumeration of microorganism contamination of the macrolide antibiotic, Spiramycin®, using epifluorescence with the ChemScan® solid phase cytometer. The artificial spiking of Spiramycin powder antibiotic with pure culture of four microorganisms led to complete recovery of the tested organisms, whatever their sensitivity to the bacteriostatic activity of the drug. With the conventional plate method run in parallel, complete recovery was only obtained for Spiramycin resistant organisms. The spiked microorganisms that were sensitive to the antibiotic remained inhibited or stressed by the action of the Spiramycin and did not grow on the plate. ER -