RT Journal Article
SR Electronic
T1 Interferon α-2a interactions on Glass Vial Surfaces Measured by Atomic Force Microscopy
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 78
OP 89
VO 56
IS 2
A1 Monica S. Schwarzenbach
A1 Peter Reimann
A1 Verena Thommen
A1 Martin Hegner
A1 Marco Mumenthaler
A1 Jacky Schwob
A1 Hans-Joachim Güntherodt
YR 2002
UL http://journal.pda.org/content/56/2/78.abstract
AB Atomic force microscopy was used to study adsorption and adhesion peculiarities of interferon α-2a on glass and mica surfaces. The specific protein adsorption behavior as a function of the pH value was illustrated on mica by single molecule imaging, while adhesion forces between interferon molecules and inner surfaces of borosilicate glass vials were measured directly under aqueous buffer conditions by force microscopy. We found that the adhesion force on Schott FIOLAX Type I plus® was reduced by 40% of the total adhesion force measured on Schott FIOLAX®, a standard type I borosilicate glass quality. These results reflect the anticipated superiority of the special “Type I plus” coating over undesired protein adsorption to glass. In addition, this study gives insight into a new method to predict unintended protein adsorption to glass container walls and to characterize the adsorption process by force measurement.