TY - JOUR T1 - Interferon α-2a interactions on Glass Vial Surfaces Measured by Atomic Force Microscopy JF - PDA Journal of Pharmaceutical Science and Technology JO - PDA J Pharm Sci Technol SP - 78 LP - 89 VL - 56 IS - 2 AU - Monica S. Schwarzenbach AU - Peter Reimann AU - Verena Thommen AU - Martin Hegner AU - Marco Mumenthaler AU - Jacky Schwob AU - Hans-Joachim Güntherodt Y1 - 2002/03/01 UR - http://journal.pda.org/content/56/2/78.abstract N2 - Atomic force microscopy was used to study adsorption and adhesion peculiarities of interferon α-2a on glass and mica surfaces. The specific protein adsorption behavior as a function of the pH value was illustrated on mica by single molecule imaging, while adhesion forces between interferon molecules and inner surfaces of borosilicate glass vials were measured directly under aqueous buffer conditions by force microscopy. We found that the adhesion force on Schott FIOLAX Type I plus® was reduced by 40% of the total adhesion force measured on Schott FIOLAX®, a standard type I borosilicate glass quality. These results reflect the anticipated superiority of the special “Type I plus” coating over undesired protein adsorption to glass. In addition, this study gives insight into a new method to predict unintended protein adsorption to glass container walls and to characterize the adsorption process by force measurement. ER -