RT Journal Article
SR Electronic
T1 Identification of Fungi Based on the Nucleotide Sequence Homology of Their Internal Transcribed Spacer 1 (ITS1) Region
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 90
OP 98
VO 56
IS 2
A1 Shoji Narutaki
A1 Kosuke Takatori
A1 Hidekatsu Nishimura
A1 Hiroshi Terashima
A1 Tsuguo Sasaki
YR 2002
UL http://journal.pda.org/content/56/2/90.abstract
AB In this study, we examined the identification of fungi based on the sequence homology of the internal transcribed spacer 1 (ITS1) region. A newly designed primer pair could amplify the target region of all 42 strains tested. The PCR products were sequenced and the sequence homologies were searched by BLAST. It was demonstrated that this method is a reliable identification method at the genus or species level. At present, available databases are still insufficient to identify some fungi, but with the accumulation of further data in the ITS1 database, this method will be available for the identification of fungi.