PT  - JOURNAL ARTICLE
AU  - Fujii, Shinji
AU  - Takai, Masaki
AU  - Maki, Takehiko
TI  - Wet Heat Inactivation of Lipopolysaccharide from <em>E. coli</em> Serotype 055:B5
DP  - 2002 Jul 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 220--227
VI  - 56
IP  - 4
4099  - http://journal.pda.org/content/56/4/220.short
4100  - http://journal.pda.org/content/56/4/220.full
SO  - PDA J Pharm Sci Technol2002 Jul 01; 56
AB  - Wet heat inactivation of lipopolysaccharide (LPS) from E. coli Serotype 055:B5 was investigated. The LPS solutions were heated at study temperatures ranging from 78°C to 175°C, and were assayed using the Limulus Amebocyte Lysate (LAL) test. Plots of the log of the amount of endotoxin remaining versus heating time showed biphasic decreases. The initial slopes are associated with a faster rate of decrease to about a 0.5-log unit reduction, and were followed by a slower, linear rate of decline in the secondary slopes. The curves were applied to the biexponential model expressed by the equation (Et = E1e−k1t + E2e−k2t). The LPS inactivation rates (k1 and k2) each conformed to their own Arrhenius equation. Therefore, the processes required to achieve the desired level of LPS inactivation can be obtained by mathematical means.