PT  - JOURNAL ARTICLE
AU  - Cai-Hong Zheng
AU  - Jian-Qing Gao
AU  - Wen-Quan Liang
AU  - He-Yong Yu
AU  - Yi-Li Zhang
TI  - Effects of Additives and Processing Parameters on the Initial Burst Release of Protein from Poly(lactic-co-glycolic acid) Microspheres
DP  - 2006 Jan 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 54--59
VI  - 60
IP  - 1
4099  - http://journal.pda.org/content/60/1/54.short
4100  - http://journal.pda.org/content/60/1/54.full
SO  - PDA J Pharm Sci Technol2006 Jan 01; 60
AB  - The aim of this study is to investigate both the effects of hydrophilic additives and combined processing parameters on the in vitro release of a model protein, bovine serum albumin (BSA), from poly(lactic-co-glycolic acid) (PLGA) microspheres. Additives including β-cyclodextrin, HP-β-cyclodextrin, poly(ethylene glycol) (PEG) 6000, and sorbitol, and processing parameters such as the poly(vinyl alcohol) (PVA) concentration, emulsification temperature, aqueous/oil phase, evaporation method, and dehydration method were evaluated. PLGA microspheres were all prepared by the double-emulsion solvent extraction/evaporation method, and the results showed that no statistically significant differences of particle sizes and entrapment efficiencies appeared. Interestingly, the initial burst releases were markedly changed by both additives and processing parameters. Initial burst releases were accelerated by hydrophilic additives except for PEG 6000 and were retarded by the formulation composed of higher PVA concentration, tween-20 as an emulsifier in the internal aqueous phase, glycerol in the oil phase, and inorganic salt in the external aqueous phase, and operated at low temperature. Scanning electron microscopy showed that the more porous and dimpled the structure on the surface of the PLGA microspheres, the larger the initial burst release. The microspheres that displayed a relatively smooth and compact surface showed the least burst release.