PT - JOURNAL ARTICLE AU - Ramesh Gannu AU - Y. Vamshi Vishnu AU - V. Kishan AU - Y. Madhusudan Rao TI - <em>In vitro</em> Permeation of Carvedilol through Porcine Skin: Effect of Vehicles and Penetration Enhancers DP - 2008 Jul 01 TA - PDA Journal of Pharmaceutical Science and Technology PG - 256--263 VI - 62 IP - 4 4099 - http://journal.pda.org/content/62/4/256.short 4100 - http://journal.pda.org/content/62/4/256.full SO - PDA J Pharm Sci Technol2008 Jul 01; 62 AB - This investigation studied the effect of vehicles on the in vitro permeation of carvedilol from saturated solutions across porcine skin and selected appropriate penetration enhancers. Labrasol, Transcutol, polyethylene glycol 400, propylene glycol, ethanol, oleic acid, isopropyl myristate, and phosphate buffered saline (pH 7.4) containing 40% v/v polyethylene glycol 400 as control, were used as vehicles; limonene, carvone, camphor, menthol, Transcutol, and Labrasol at 5% w/v concentrations were used as penetration enhancers. Skin permeation studies were conducted in Franz diffusion cells using excised porcine ear skin. Solubility was highest (369.13 mg/mL) in Transcutol, whereas isopropyl myristate showed the lowest solubility (0.79 mg/mL) among all the vehicles. The flux of carvedilol from Transcutol, Labrasol, polyethylene glycol 400, ethanol, and oleic acid was 10.5, 8.6, 4.2, 2.9, and 1.5 times higher, respectively, than that observed with control. The flux obtained using Transcutol was significantly higher (P &lt; 0.05) than the flux obtained using the other vehicles. However, the flux values of carvedilol using isopropyl myristate (P &lt; 0.01) and propylene glycol (P &lt; 0.05) were significantly lower than that of the control. Solutions containing 5% w/v camphor showed maximum permeation (232.54 μg) in 24 h with a flux of 3.19 μg/cm2/h, which was significantly different (P &lt; 0.05) than the flux obtained using other permeation enhancers. The control sample showed lowest permeation (30.50 μg), with a flux of 0.33 μg/cm2/h. The flux of carvedilol from the solutions containing 5% w/v camphor, limonene, Transcutol, carvone, Labrasol, and menthol were 9.7, 7.6, 7.6, 6.3, 4.7, and 2.3 times higher, respectively, than that observed using the control. The present study suggests that Transcutol, Labrasol, and polyethylene glycol 400 may be used as potential vehicles and camphor, limonene, and Transcutol at a 5% w/v level as penetration enhancers.