RT Journal Article
SR Electronic
T1 Preparation and Stability of Diallyl Trisulfide Self-Assembled Micellar Injection
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 92
OP 96
VO 64
IS 2
A1 Xiulan Ju
A1 Shixuan Zhang
A1 Qing Wang
A1 Xiaona Li
A1 Puwen Yang
YR 2010
UL http://journal.pda.org/content/64/2/92.abstract
AB Diallyl trisulfide (DATS) has shown various biological activities, such as antimicrobial, antihyperlipidemic, antithrombotic, and anticancer effects. It is a highly water insoluble thioether compound, which irritates blood vessels and degrades rapidly under normal conditions. Hence, a suitable DATS delivery system and the related stability studies are both valuable for the clinical use of DATS. The DATS micellar injection in the present study was prepared using propylene glycol, alcohol, Tween 80, and water by the self-assembling micellar method, which effectively avoided irritation and degradation of DATS. The high-pressure liquid chromatography-UV assay method was established and validated for the quantitative determination of DATS micellar injection. The major degraded compounds were identified as diallyl disulfide and diallyl tetrasulfide by gas chromatography–mass spectrometry. 3-ethenyl-3,6-dihydro-1,2-dithiin and 3-ethenyl-3,4-dihydro-1,2-dithiin might be the thermal decomposed products of DATS in process of gas chromatography–flame ionization detector analysis. The main factor influencing stability in DATS micellar injection was ascertained to be temperature. This investigation might be a beneficial contribution to further identification of DATS-degraded products and exploration of the delivery system of DATS.© PDA, Inc. 2010