PT  - JOURNAL ARTICLE
AU  - Varmette, Elizabeth
AU  - Strony, Brianne
AU  - Haines, Daniel
AU  - Redkar, Rajendra
TI  - An Assay for Measurement of Protein Adsorption to Glass Vials
DP  - 2010 Jul 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 305--315
VI  - 64
IP  - 4
4099  - http://journal.pda.org/content/64/4/305.short
4100  - http://journal.pda.org/content/64/4/305.full
SO  - PDA J Pharm Sci Technol2010 Jul 01; 64
AB  - Protein adsorption to primary packaging is one of the problems faced by biopharmaceutical drug companies. An assay was developed to quantify loss of proteins to glass vial surfaces. The assay involves the labeling of protein with a fluorescent dye, incubation of the labeled protein with the vial surface, elution of the adsorbed protein using a stripping buffer, and determination of fluorescence of the adsorbed protein using a fluorometer. The assay is simple to set up, accurate, sensitive, and flexible. The assay can be modified for indirect measurement of protein adsorption and offers an attractive alternative for researchers to quantify protein adsorption to glass vials and syringes.