RT Journal Article
SR Electronic
T1 An Assay for Measurement of Protein Adsorption to Glass Vials
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 305
OP 315
VO 64
IS 4
A1 Varmette, Elizabeth
A1 Strony, Brianne
A1 Haines, Daniel
A1 Redkar, Rajendra
YR 2010
UL http://journal.pda.org/content/64/4/305.abstract
AB Protein adsorption to primary packaging is one of the problems faced by biopharmaceutical drug companies. An assay was developed to quantify loss of proteins to glass vial surfaces. The assay involves the labeling of protein with a fluorescent dye, incubation of the labeled protein with the vial surface, elution of the adsorbed protein using a stripping buffer, and determination of fluorescence of the adsorbed protein using a fluorometer. The assay is simple to set up, accurate, sensitive, and flexible. The assay can be modified for indirect measurement of protein adsorption and offers an attractive alternative for researchers to quantify protein adsorption to glass vials and syringes.