PT  - JOURNAL ARTICLE
AU  - Michael J Rubino
TI  - Experiences with HEK293: A Human Cell Line
DP  - 2010 Sep 01
TA  - PDA Journal of Pharmaceutical Science and Technology
PG  - 392--395
VI  - 64
IP  - 5
4099  - http://journal.pda.org/content/64/5/392.short
4100  - http://journal.pda.org/content/64/5/392.full
SO  - PDA J Pharm Sci Technol2010 Sep 01; 64
AB  - The paper describes considerations taken with regard to a recombinant human cell line (HEK293) producing a therapeutic protein. When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses. A viral contamination event occurred with this cell line even after a number of precautions were taken.