RT Journal Article
SR Electronic
T1 Experiences with HEK293: A Human Cell Line
JF PDA Journal of Pharmaceutical Science and Technology
JO PDA J Pharm Sci Technol
FD Parenteral Drug Association (PDA)
SP 392
OP 395
VO 64
IS 5
A1 Michael J Rubino
YR 2010
UL http://journal.pda.org/content/64/5/392.abstract
AB The paper describes considerations taken with regard to a recombinant human cell line (HEK293) producing a therapeutic protein. When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses. A viral contamination event occurred with this cell line even after a number of precautions were taken.